megatomi.com Fundamentals Explained
megatomi.com Fundamentals Explained
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Speedily receive sections as slender or thick as your software needs, devoid of worrying about dropping or distorting precious tissue samples.
Megatome is a vibrating microtome intended to part a wide number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables large-throughput tissue sectioning with uniform floor profile, and also small tissue destruction and information loss.
Antibodies might get weeks to diffuse via only a few millimeters of tissue, using a steep labeling gradient from floor to Main.
Megatome is created for accuracy: the blade vibrates at a better frequency and larger amplitude assortment than other microtomes, and includes a special deflection Command system.
SE makes use of a rotational electric field to disperse highly electromobile molecules (which include antibodies or surfactant micelles) in the course of a porous sample devoid of detrimental electrically charged structures inside the tissue. This permits 2-four working day clearing of intact organs,
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Megatome is actually a novel microtome that allows for top-precision sectioning of an array of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with minimal tissue problems and information decline.
eFLASH can be a rapid tissue labeling procedure that permits for uniform full-organ staining in 20 rounds of labeling.
The SmartSPIM light sheet microscope supplies unparalleled resolution and acquisition speed in the course of intact tissue volumes, whilst its modular structure permits you to enhance and personalize unique elements.
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SHIELD avoids the variability of hydrogel embedding and the knowledge decline from PFA preservation, protecting specimens for several rounds of processing.
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Our novel Defend tissue preservation procedure sorts intramolecular bonds using polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.